The CytoSinct CD34 Nanobeads human are used for in vitro enrichment CD34 cells from fresh or frozen peripheral blood mononuclear cells (PBMCs) leukapheresis products or single cell suspension based on the surface expression of human CD34
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CytoSinct CD34 Nanobeads human (GMP) is intended for the in vitro magnetic labeling for pre-separation or other processing of human CD34 positive cells from heterogeneous cell populations enabling further cultivation and/or genetic modification of the cells
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Mononuclear cells are separated from umbilical cord blood by means of a density gradient centrifugation protocol. Human cord blood CD34 depleted mononuclear cells are isolated from the mononuclear cell fraction by removing the CD34+ population by immunomagnetic separation. The CD34 depleted mononuclear cells contain less than 0.1% CD34+ cells. The presence of CD34+ cells is assessed by flow cytometry prior to cryopreservation. The isolated MNCs can be used to isolate subpopulations or used as a control for donor matched CD34+ stem cell experiments (Please request the CD34 depleted flow-through fraction upon ordering CD34+ stem cells).
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Primary bone marrow mononuclear cells (a.k.a. BM MNCs or BMNCs) are a heterogeneous population that includes hematopoietic lineage cells such as lymphocytes, monocytes, stem cells, and progenitor cells as well as mesenchymal stromal cells. Mononuclear cells are isolated from human adult bone marrow using density gradient separation. Heparin is added to the bone marrow upon collection as an anticoagulant.Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.
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Normal Human Dermal Microvascular Endothelial Cells (HD-MVECn), when grown in Lifeline's VascuLife Medium, provides an ideal culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis, or vascular biology.
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Lifeline's normal Human Lung Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.
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Normal Human Dermal Fibroblasts-Neonatal, Xeno-Free, when grown in Lifeline's FibroLife Xeno-Free Complete Medium, provide an ideal xeno-free culture system to establish xeno-free human feeder layers for human embryonic stem cell cultures.
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